Extraction of Dna from Human Saliva and Blood and Genetic Analysis of Str Markers-a Comparative Study

Sucheta Bansal, S. K. Joshi 1-DEPARTMENT OF ORAL AND MAXILLOFACIAL PATHOLOGY; 2DEPARTMENT OF PROSTHODONTICS INCLUDING CROWN AND BRIDGE & IMPLANTOLOGY, HIMACHAL INSTITUTE OF DENTAL SCIENCES PAONTA SAHIB, HIMACHAL PRADESH, INDIA Summary As a diagnostic fluid, saliva offers many advantages over serum especially in forensic science as it is good source for DNA retrieval which could be used for individualization. The objective of this study was to evaluate the use of salivary DNA for amplification of Short tandem repeats (STRs) and its comparison with same STRs in blood DNA. Blood and Saliva samples were collected from 20 patients reporting in Himachal Institute of Dental Sciences, Paonta Sahib, Himachal Pardesh, after informed consent and approval from Ethics Committee. Extraction of DNA was done through Phenol Chloroform method both in blood and saliva. PCR was used for amplification of 2 Short tandem repeats (STR), Amelogenin (AMG) and von Willebrand factor (vWF) which were than matched with blood. On amplification of AMG in blood all 20 samples (100%) amplified, whereas in case of saliva, out of 20 samples 17 samples (85%) amplified. Out of these 17 samples 16 samples (94.1%) of salivary DNA matched with amplified samples of blood DNA. For the vWF gene all 10 samples (50%) which amplified, matched positively with blood DNA. Based on the obtained results, it may be concluded that amplification of AMG from salivary DNA samples have a high potential and is not inferior to blood.